Transient partial permeabilization with saponin enables cellular barcoding prior to surface marker staining

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Transient partial permeabilization with saponin enables cellular barcoding prior to surface marker staining.

Fluorescent cellular barcoding and mass-tag cellular barcoding are cytometric methods that enable high sample throughput, minimize inter-sample variation, and reduce reagent consumption. Previously employed barcoding protocols require that barcoding be performed after surface marker staining, complicating combining the technique with measurement of alcohol-sensitive surface epitopes. This repor...

متن کامل

Saponin-permeabilization is not a viable alternative to isolated mitochondria for assessing oxidative metabolism in hibernation

Saponin permeabilization of tissue slices is increasingly popular for characterizing mitochondrial function largely because it is fast, easy, requires little tissue and leaves much of the cell intact. This technique is well described for mammalian muscle and brain, but not for liver. We sought to evaluate how saponin permeabilization reflects aspects of liver energy metabolism typically assesse...

متن کامل

Multiple Hypotheses Testing with Partial Prior Information

By modifying the optimal criterion of Kudo (1967), an alternative theory is developed for incorporating prior probabilities into multiple hypotheses testing. Unlike Kudo’s theory, an explicit optimal test can be obtained. It is shown that the optimal test is a natural extension of some well-known tests such as the maximum likelihood test.

متن کامل

Efficient Partial Monitoring with Prior Information

Partial monitoring is a general model for online learning with limited feedback: a learner chooses actions in a sequential manner while an opponent chooses outcomes. In every round, the learner suffers some loss and receives some feedback based on the action and the outcome. The goal of the learner is to minimize her cumulative loss. Applications range from dynamic pricing to label-efficient pr...

متن کامل

Experimental quantum cloning with prior partial information.

When prior partial information about a state to be cloned is available, it can be cloned with a fidelity higher than that of universal quantum cloning. We experimentally verify this intriguing relationship between the cloning fidelity and the prior information by reporting the first experimental optimal quantum state-dependent cloner, using nuclear magnetic resonance techniques. Our experiments...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Cytometry Part A

سال: 2014

ISSN: 1552-4922

DOI: 10.1002/cyto.a.22573